rabbit anti vaccinia virus serum  (Cedarlane)

Journal: PLoS ONE

Article Title: Genomic Expression Libraries for the Identification of Cross-Reactive Orthopoxvirus Antigens

doi: 10.1371/journal.pone.0021950

Figure Lengend Snippet: For a validation of their complexity the constructed genomic EL were serologically screened with polyclonal and monoclonal antibodies of known specificities. Immunopositive signals are exemplary indicated through white arrows for the following screening combinations: (A) EL-CPXV-0.2k-0.7k with polyclonal goat anti-rA27 serum, (B) EL-CPXV-0.2k-3k with monoclonal mouse anti-rA27 antibody, (C) EL-VACV-3k-12k with goat anti-rA27, and (D) EL-VACV-0.2k-0.7k with goat anti-rA27. Further evaluation was performed by immunoscreening a genomic CPXV EL with different sera and controls: (E) Immunoscreening of EL-CPXV-0.2k-3k using serum from a VACV-immunized rabbit. The white arrow points to an immunopositive plaque, the black arrow to an immunonegative plaque. (F) Goat anti-rabbit IgG conjugate alone without primary antibody. (G) Human serum from a poxvirus-naive person. (H) Human poxvirus-naive anti-dengue virus serum.

Article Snippet: The hyperimmune rabbit anti-VACV (strain Lister) serum was obtained from Acris Antibodies GmbH (Herford, Germany).

Techniques: Construct

Journal: PLoS ONE

Article Title: Genomic Expression Libraries for the Identification of Cross-Reactive Orthopoxvirus Antigens

doi: 10.1371/journal.pone.0021950

Figure Lengend Snippet: The main circle represents the entity of identified immunogenic OPV proteins. This set of proteins is divided into four subsets depending on the experimental setting: 1. CPXV EL screened with anti-VACV sera; 2. CPXV EL screened with anti-CPXV sera; 3. VACV EL screened with anti-VACV sera; and 4. VACV EL screened with anti-CPXV sera. Subsets 1 and 4 contain cross-reactive antigens. The inner circles represent the intersection of identified antigens. Thereby a differentiation is made between the same protein being present in two different subsets and a protein being present in all four subsets (central circle).

Article Snippet: The hyperimmune rabbit anti-VACV (strain Lister) serum was obtained from Acris Antibodies GmbH (Herford, Germany).

Techniques:

Journal: Viruses

Article Title: Protective Effect of Surfactant Protein D in Pulmonary Vaccinia Virus Infection: Implication of A27 Viral Protein

doi: 10.3390/v5030928

Figure Lengend Snippet: Binding of surfactant proteins to Vaccinia virus (VACV). Three VACV strains, VACV-WR, VACV-Lister and VACV-IHD-J, were dotted onto membranes and incubated with 2 µg of various surfactant proteins. After extensive washes, bound SPs were detected with specific anti-surfactant protein antibodies and revealed by enhanced chemiluminescence (ECL). Mock membrane was incubated with specific anti-SP antibodies. RhSP-D and RhSP-A, dotted onto SP control membrane, were revealed with specific anti-SP antibodies. AP-SP-A, human SP-A from alveolar proteinosis; RhSP-A, recombinant human SP-A; RhSP-D, recombinant human SP-D.

Article Snippet: Rabbit anti-vaccinia whole virus antibody was purchased from Fitzgerald-Interchim (20VR-69) and both peroxydase-conjugated goat anti-rabbit and anti-mouse IgG antibodies were purchased from Jackson ImmunoResearch.

Techniques: Binding Assay, Incubation, Recombinant